Journal: Diagnostic Pathology
Article Title: Intraventricular glioneuronal tumor with disseminated lesions at diagnosis - a case report -
doi: 10.1186/1746-1596-6-119
Figure Lengend Snippet: Photomicrographs showing immunohistochemistry for neuronal markers . (a) neuron-specific enolase (NSE), (b) microtubule-associated protein-2 (MAP-2), (c) Synaptophysin, (d) neuronal nuclear antigen (NeuN), (e) tubulin, βIII isoform (TuJ1). The markers in a-d were diffusely positive in the part of the tumor with neuronal differentiation. However, TuJ1 was only weakly positive there. Olig2-positive cells were also observed in the same area (f). The cells comprising the perivascular pseudorosette were strongly positive for NSE (g) and MAP-2 (h), a-f: ×100; g and h: ×200.
Article Snippet: The primary antibodies and their dilution with buffer were as follows: rabbit polyclonal anti-olig2 antibody (1:200; Millipore, Temecula, CA), polyclonal anti-vimentin antibody (1:200; Dako, Glostrup, Denmark), mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (1:500; Dako), mouse monoclonal anti-synaptophysin (Syn) antibody (1:50; Millipore), mouse monoclonal anti-tubulin, βIII isoform (TuJ1) antibody (1:200; Millipore), monoclonal anti-neuronal nuclear antigen (Neu-N) antibody (1:100; Millipore), monoclonal anti-microtubule associated protein-2 (MAP-2) antibody (1:100; Millipore), monoclonal anti-neuron-specific enolase (NSE) and monoclonal anti-Ki-67/MIB-1 antibody (1:50; Dako).
Techniques: Immunohistochemistry